Did You Know Just That Many Cancers Are Linked To A Vitamin Deficiency?
Liquid
vitamin B, in PubMed
Liquid chromatographic
analysis of vitamin B6 in reconstituted infant formula:
collaborative study.
Mann DL, Ware GM,
Bonnin E, Eitenmiller RR, Barna E, Christiansen S, De Borde
JL, DeVries J, Gilliland P, Hemmer J, Kalman A, Konings
E, Levin D, Salvati L, Woollard D.
U.S. Food and Drug
Administration, Southeast Regional Laboratory, 60 Eighth
St, NE, Atlanta, GA 30309, USA.
A liquid chromatographic
(LC) method was validated for the determination of total
vitamin B6 in infant formula. Total vitamin B6 was quantified
by converting the phosphorylated and free vitamers into
pyridoxine. Pyridoxine was determined by ion pair reversed-phase
LC with fluorescence detection. The method was subjected
to an AOAC collaborative study involving a factory-manufactured,
milk- and soy-based infant formula. Each was spiked at 3
concentrations in the range of 0-1 microg/g and sent as
blind duplicate to participant laboratories. Nine laboratories
returned valid data which were statistically analyzed for
outliers and precision parameters. The repeatability relative
standard deviation (RSD(r)) ranges were 2.0-4.0 and 3.5-5.9%
for fortified milk- and soy-based formulas, respectively.
The reproducibility relative standard deviation (RSD(R))
ranges were 8.2-8.4 and 6.7-11.2% for fortified milk- and
soy-based formulas, respectively. HORRAT values ranged from
0.42 to 0.53, indicating that the precision of the method
is acceptable. The mean RSD(r):RSD(R) values were 0.60 and
0.55 for milk- and soy-based formulas, respectively. As
expected, RSDs for the unfortified samples were higher,
but their HORRAT values (0.81 and 2.06) helped define a
realistic limit of quantitation as 0.05 microg/g. Recovery
data were quantitative and varied between 81.4 and 98.0%
(mean = 89.8%) for each of 6 spiked materials.
PMID:
15759723 [PubMed - indexed for MEDLINE]
http://www.ncbi.nlm.nih.gov/entrez/
query.fcgi?cmd=Retrieve&db=
PubMed&list_uids
=15759723&dopt=Abstract
